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FRI0374 (2020)
PLASMA LEVELS OF 14-3-3 PROTEIN, S100A8/S100A9-PROTEIN, INTERLEUKIN-6, INTERLEUKIN-18, INTERLEUKIN-4, INTERLEUKIN-17, INTERLEUKIN-1Β AND TUMOR NECROSIS FACTOR-Α IN CHRONIC NON-BACTERIAL OSTEOMYEILITIS AND NON-SYSTEMIC JUVENILE IDIOPATHIC ARTHRITIS
M. Kostik1,2, M. Makhova3, D. Kozlova4,5, D. Vasilyev4,6, L. Sorokina2,7, E. Isupova1,8, A. Maletin9, A. Mushkin9
1Saint Petersburg State Pediatric Medical University, Saint Petersburg, Russian Federation
2Almazov National Medical Research Centre, Saint-Petersburg, Russian Federation
3Saint Petersburg State Pediatric Medical University, Saint-Petersburg, Russian Federation
4LLC Scientific and Production Company “ABRIS+”, Saint-Petersburg, Russian Federation
5Sechenov Iinstitute of Evolutionary Physiology and Biochemistry, Saint-Petersburg, Russian Federation
6Sechenov Institute of Evolutionary Physiology and Biochemistry, Saint Petersburg, Russian Federation
7Leningrad Regional Children’s Hospital, Saint Petersburg, Russian Federation
8Saint Petersburg, Saint Petersburg, Russian Federation
9Science Research Institute of Phthisiopulmonology, Saint Petersburg, Russian Federation

Background: Chronic non-bacterial osteomyelitis (CNO) is an immune-mediated disease associated with cytokine dysbalance.


Objectives: The aim of our study was to evaluate the cytokines levels in CNO and compare to juvenile idiopathic arthritis (JIA) – disease with immune-mediated mechanism.


Methods: The diagnosis of CNO made with criteria, proposed by Jansson (2007, 2009), after the exclusion of other causes of bone disease [1]. We included 42 patients with NBO, 28 patients with non-systemic juvenile idiopathic arthritis (JIA). We evaluated plasma levels of 14-3-3 protein, S100A8/S100A9-protein, interleukine-6 (IL-6), interleukine-18 (IL-18), interleukine-4 (IL-4), interleukine-17 (IL-17), interleukine-1β (IL-1 β) and tumor necrosis factor-α (TNFα) in 2 groups by the ELISA. Statistical analysis was carried out with Statistica 10.0 software. We utilized descriptive statistics (Me; IQR), Mann-Whitney tests.


Results: We have found differences in the proinflammatory biomarkers between CNO, JIA. Patients with NBO had lower levels of studied cytokines, exclude14-3-3-protein, S100A8/S100A9 and interleukin-6 compare to JIA patients ( table 1 ).

Comparison the cytokine levels between CNO, JIA N

Parameter NBO (n=42) JIA (n=28) p
Hemoglobin, g/l 112 (104; 124) 120 (114.5; 126.0) 0.02
WBC x 10 9 /l 7.9 (7.0; 10.5) 8.0 (6.7; 10.0) 0.86
PLT x 10 9 /l 347 (259; 408) 336.5 (274.0; 390.5) 0.98
ESR. mm/h 25.0 (9.0; 46.0) 8.5 (2.5; 13.0) 0.013
CRP, mg/l 6.1 (0.6; 2.4) 1.8 (0.4; 11.9) 0.027
14-3-3, ng/ml 21.4 (18.5; 27.1) 19.9 (18.0; 27.8) 0.77
S100A8/S100A9, ng/ml 5.9 (5.2; 6.5) 5.9 (5.0; 6.2) 0.76
IL-6, ng/ml 126,2 (112.8; 137.5) 132.4 (117.4; 142.9) 0.16
IL-18, ng/ml 270.1 (200.1; 316.1) 388.3 (373.9; 405.1) 0.0000001
IL-4, ng/ml 15.3 (11.5; 18.2) 18.7 (16.2; 20.2) 0.003
IL-17, ng/ml 83.1 (71.1; 97.3) 99.2 (87.3; 115.8) 0.003
IL-1b, ng/ml 47.4 (42.0; 51.3) 70.8 (65.3; 73.6) 0.0000001
TNFa, ng/ml 19.4 (17.8; 21.3) 23.1 (20.2; 25.9) 0.0006

Conclusion: Patients with CNO had less proinflammatory activity then JIA patients, besides IL-6 and S100A8/S100A9. Further investigations required for finding new more precise biomarkers and finding possible molecular targets for treatment.

This work supported by the Russian Foundation for Basic Research (grant № 18-515-57001)


REFERENCES:

[1]Jansson AF, et al. Clinical score for nonbacterial osteitis in children and adults. Arthritis Rheum. 2009;60(4):1152-9.


Disclosure of Interests: None declared

Citation: Ann Rheum Dis, volume 79, supplement 1, year 2020, page 780
Session: Bone diseases, including osteoporosis and osteoimmunology: aetiology, pathology and animal models (Poster Presentations)